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病毒检测系列

Zika Virus NS1 ELISA Kit
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商品描述

BioFront Technologies now offers the very first ELISA kit that specifically detects Zika virus NS1 protein! Detect all the major ZIKV genotypes with no cross-reactivity with Dengue virus NS1. Avoid time consuming RNA extraction steps required for RT-PCR and directly quantify intracellular NS1 or secreted NS1 in plasma and cell culture supernatants in under 2 hours!

 

Item #:ZIKV-NS1-EK-192

Description: Zika Virus NS1 ELISA Kit- 2 X 96-well plates allows users to test up to 192 samples

Intended Use (please note, this kit is currently not validated for clinical use):

The MonoTrace Zika virus (ZIKV) non-structural protein 1 (NS1) enzyme-linked immunosorbent assay (ELISA) offers researchers a fast, affordable, and accurate way to detect the presence of the ZIKV in vitro. The assay can detect ZIKV NS1 present in cell culture supernatants or cell lysates and saves time compared to Western blotting (quantifiable results in under 2 hours) while offering high-throughput yet cost-efficient alternative to RT-qPCR.

zika virus ELISA

Left: Standard curve generated using recombinant Zika Virus (rZIKV) NS1 protein
Right: Detection of secreted ZiKV NS1 protein in titered cell culture supernatant

The highly sensitive monoclonal antibody-based sandwich ELISA recognizes the 3 major ZIKV genotypes and can specifically detect ZIKV NS1 without cross-reactivity to Dengue virus.

Left: Specific detection of secreted ZIKV NS1 protein in supernatant from Zika virus infected cells (ZKV). Supernatant from uninfected (mock) or Dengue virus infected (DENV) cells were negative.
Right: Specific detection of intracellular ZKV NS1 protein in lysates from Zika virus infected cells (ZKV). Lysates from uninfected (mock) or Dengue virus infected (DENV) cells were negative.

With no expensive equipment required and an assay time of less than 2 hours, the MonoTrace Zika Virus NS1 ELISA is the perfect tool for monitoring infection or screening antiviral therapeutics.

 

Specificity: Antibodies employed in this sandwich ELISA recognize conserved epitopes on the ZIKV NS1 protein and show no cross-reactivity to Dengue virus NS1

 

Form: Two (2) monoclonal antibody coated 96-well plates come in convenient removable strip format (12 X 8-well strips per plate)

 

Components:

1. Anti-ZIKV NS1 monoclonal antibody coated 96-well plate = 2 plates

2. 10X NS1 lysis buffer = 25 ml

3. 5X sample diluent = 25 ml

4. 10X Wash Buffer = 25 ml

5. Ready-to-use full-length recombinant NS1 standards (no titration required) = 2 ml each

6. HRP-conjugated anti-NS1 detection antibody = 2 X 12 ml

7. High sensitivy substrate solution = 2 X 12 ml

8. HRP quench solution =2 X 12 ml

Performance: Range of quantification = 0.2 - 16 ng/ml Zika virus NS1

 

Storage/Shelf life: Each plate is packed in a vacuum-sealed Mylar(TM) pouch with desiccant packets to extend the shelf life of the product to 6 months from the date of manufacture, if kept unopened and stored at 4°C.

 

References: 

1) Xu, Lee, Wen et al. Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen. Nature Medicine (2016). doi:10.1038/nm.4184.
2) Larocca et al. Vaccine protection against Zika virus from Brazil. Nature (2016). doi:10.1038/nature18952.

 

 

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