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过敏原试剂盒

Crustacea ELISA Kit
  现 价: ¥0元
  产品规格:
48孔
96孔
  库 存 量: 9件
  快递价格: 20元
  销 售 量: 8件
  购买数量:
商品描述

MONOTRACE CRUSTACEAN ELISA KIT-48/96 wells 虾蟹过敏原试剂盒

Under the US Food Allergen Labeling and Consumer Protection Act, crustaceans (shellfish) are among the eight foods or food groups designated as "major food allergens" by congress. Allergies to shellfish occur primarily from the muscle protein tropomyosin in raw and cooked food and often persist throughout adult life, making BioFront's MonoTrace Crustacean ELISA kit a critical food safety diagnostic tool for the protection of shellfish sensitive individuals.

CRUSTACEAN  ASSAY PERFORMANCE 虾蟹试剂盒性能分析

Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 48 or 96

ITEM #: CR1-EK-48 & CR1-EK-96

*Limit of Detection: 0.07 ppm (shellfish)

Range of Quantification: 1 to 40 ppm (shellfish)

Specificity: Crustacean tropomyosin

Reactivity: Reacts equally to raw and cooked tropomyosin from shellfish, including shrimp, crab, lobster, prawn, and crayfish.

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

实验时间:现在抽提后仅需30分钟!

孵育时间:抽提,样品孵育,二抗孵育,底物孵育每步仅需10分钟。

每一试剂盒测试数量:48 & 96

*检测限:0.07 ppm(贝类)

定量范围:140 ppm(贝类)

特异性:甲壳动物 原肌球蛋白

反应:对来自贝类,包括虾,螃蟹,龙虾,虾和小龙虾的原始和煮熟的原肌球蛋白均反应。

*=参数的计算根据10次代表性的数据得出,然而,使用者应当查阅附随的分析证书中的具体批次数值。

THE MONOTRACE ADVANTAGE 优势

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1Target a major allergen resistant to food processing effects

2Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility of ‘false positive’ results

3Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies开发了第一条完全以单克隆抗体为基础的检测试剂盒产品(MonoTrace ELISAs),检测各种食物基质中的致敏蛋白污染。MonoTrace ELISAs综合使用一系列精准确定的经过严格筛选过程后仔细挑选的单克隆抗体来实现:

1、分析耐食物加工的主要过敏原

2、特异性识别目标成分,从而消除交叉反应,以及减少“假阳性”结果的可能性

3、在各种食品(食品基质干扰可以忽略不计)的检测中表现优异

CROSS-REACTIVITY 交叉反应

The assay exhibits strong reactivity to Crustacea and to a significantly lesser extent showed crossreactivity to octopus (0.0205%). Mussels (0.0013%) demonstrated minimal cross-reactivity when tested with the MonoTrace Crustacea ELISA. At concentrations as high as 100,000 ppm, there was no cross reactivity to tree nuts, legumes, vegetables, seeds, flours or meats. Reactivity towards raw and cooked shrimp, prawn, lobster, crayfish and crab was similar.

RECOVERY 回收

The MonoTrace Crustacea ELISA kit is suitable for the qualitative or quantitative detection of Crustacea within wine, soup mix, fish batter mix, sauces, tomato juice, imitation crab and sesame oil. Recoveries were acceptable, according to current AOAC guidelines (J. AOAC Int. 2012). In addition, Crustacea was efficiently recovered from shrimp-incurred fish ball samples that were cooked under varying conditions. Commercial samples were evaluated with the MonoTrace Crustacea ELISA. All commercial samples labeled as containing shrimp and/or crab were detected. Lobster was not detected within lobster stock but it was detected within the bisque. In conclusion, raw and cooked Crustacea was efficiently extracted and detected using the MonoTrace Crustacea ELISA from uncooked and cooked food matrices.

Other matrices may also be suitable for the MonoTrace Crustacea ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). An internal validation using a Crustacea-spiked sample is recommended to ensure that protein is efficiently recovered before qualitative or quantitative results are obtained from the kit.

 

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