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过敏原试剂盒

MonoTrace Gluten ELISA Kit
  现 价: ¥0元
  产品规格:
48孔
96孔
  库 存 量: 10件
  快递价格: 20元
  销 售 量: 88件
  购买数量:
商品描述

 

MONOTRACE GLUTEN ELISA KIT  - 48/96 wells 麸质过敏原试剂盒

Gluten is primarily composed of gliadin and glutenin, two abundant seed storage proteins found within wheat, barley and rye. Sensitivity to gluten affects 6% of the U.S. population and includes individuals affected by celiac disease, wheat allergy, and non-celiac gluten sensitivity. These individuals experience a range of symptoms upon ingestion of wheat and related cereals and there is a growing commercial need for new methods to rapidly and accurately detect gluten in foods for the protection gluten sensitive individuals.

Using a novel set of gluten-specific monoclonal antibodies, BioFront Technologies has developed the MonoTrace Gluten ELISA, a robust assay for detecting gluten in a wide variety of complex food systems while utilizing a novel non-toxic extraction buffer.

GLUTEN ASSAY PERFORMANCE  麸质试剂盒性能分析

Monoclonal antibodies:The MonoTrace Gluten ELISA is the first assay to incorporate the use of two new gliadin-specific monoclonal antibodies, clones 13F6 and 14F11.

Extraction: For detecting and accurately quantifying gluten contamination within raw food ingredients and processed foods,extraction from various commercial and in-house incurred samples was optimized using a new non-toxic extraction buffer, the MonoTrace Gluten Extraction Buffer (MGEB), for 1 hour at 60 C

Recovery: Recovery from various food matrices and ingredients (n=18) was analyzed using in-house incurred cornbread samples, gluten-free (GF) commercial products and ingredients, as wells as quality control and reference materials sourced from the Food Analysis Performance Assessment Scheme (FAPAS) division of the Food and Environment Research Agency (FERA). Recovery of gluten from in-house gluten-incurred cornbread and gluten-spiked commercial food matrices was >75%.

Cross-reactivity: Cross-reactivity was tested using a large panel of GF and non-GF labeled commercial samples from various vendors (n=126). No cross-reactivity was observed in any of the tree nuts, legumes, seeds, meats or spice samples tested. Wheat, rye and barley flours were highly CR. The assay also exhibits little to no cross-reactivity to several oat cultivars (n=5).

Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for sample incubation, conjugate incubation, and substrate incubation.

Number of Tests per Kit: 48 or 96

Limit of Detection:0.3 ppm gluten (0.15 ppm gliadin)

Range of Quantification: 2-100 ppm gluten (1-50 ppm gliadin)

Specificity: Gluten

 

ITEM#: GLU-EK-48 or GLU-EK-96

WHY CHOOSE THE MONOTRACE GLUTEN ELISA?

l  No hazardous waste generated!

l  Results in under 2 hours!

l  Sample kits and supporting quality control/reference materials available to help with internal validations. CLICK TO REQUEST FREE TRIAL!

 

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