通知公告: 贝若凡与您相约CBIFS2018

联系我们

 

地址:安徽省合肥市高新区

   天达路2号安大科技园

电话:186-555-66537

邮箱:sales@biofrontchina.com

病毒检测系列

Anti-HCV NS3 antibody [2E3]
  现 价: ¥3680元
  产品规格:
100ul
  库 存 量: 100件
  快递价格: 20元
  销 售 量: 18件
  购买数量:
商品描述

我公司所有抗体均为美国进口,国内分装,现全部低价促销!                                      品牌:BioFront

Clone 2E3

克隆2E3

Volume100 μl (1 μg/μl)

体积100 μl (1 μg/μl)

SpecificityRecognizes the helicase   domain of JFH-1 NS3 (genotype 2a)

特异性:识别JFH-1 (基因型2a) NS3 解旋酶片段

Cross-reactivityCross-reactive   against genotype 1a

交叉反应:与基因型1a有交叉反应

FormProtein   A purified

形式A蛋白纯化

OriginMouse

来源:小鼠

Isotype IgG1

同种型IgG1

Buffer/Composition1X PBS pH 7.2; 0.1% sodium   azide (NaN3)

缓冲液/成分1X   PBS pH 7.20.1% 叠氮化钠 (NaN3)

Storage: Short Term (<6 months): 4.  Long Term (up to 1   year): -20   (avoid repeated freeze/thaw)

储存:短期(<   6个月):4 长期(可达1年):-20(避免反复冻融)

Applications: Western Blot, IF, IP,   FACS, and TCID50 assay

应用:免疫印迹(Western),免疫荧光(IF),免疫沉淀(IP),流式细胞分析(FACS

    半数组织培养感染量分析(TCID50 assay

Working Dilutions: TCID50- 1:1000-1:5000,   Western Blot- 1:1000-1:5000, IF- 1:1000, FACS- 1:500

工作稀释度TCID50-   1:1000-1:5000, Western Blot- 1:1000-1:5000, IF- 1:1000, FACS- 1:500

References:

参考文献:

(1) Jirasko et al. PLoS Pathog. 2010 Dec 16;6(12):e1001233.

(2) Backes et al. J Virol. 2010 Jun;84(11):5775-89

(3) Kaul et al. PLoS Pathog. 2009 Aug;5(8):e1000546

(4) Yang et al. J Virol. 2008 Jun;82(11):5269-78.

Cell lysate run on 12% SDS-PAGE and transferred to PVDF.  Primary antibody incubation performed for 1-hour at room temperature (overnight incubations also suitable at 4 degrees Celsius).

 

细胞裂解物在12% SDS-PAGE电泳并转膜(PVDF)。一抗室温孵育1小时(4过夜孵育也可)。

 

REFERENCES:

参考文献:

Paul D, Hoppe S, Saher G, Krijnse-Locker J, Bartenschlager R. Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment.  J Virol. 2013 Oct;87(19):10612-27.


Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.


Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.


Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation.  PLoS Pathog 2012; 8 (4).


Nag A, Robotham JM, Tang H. Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors. J Virol. 2012 Dec;86(23):12616-24.


Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation.  Nucleic Acids Res. 2009 Oct;37(19):6587-99.


  • Item #: HCV-2E3

 

版权所有:贝若凡生物科技(合肥)有限公司 皖ICP备14016888号

地址:安徽省合肥市高新区天达路2号  邮编:230088 技术支持:一浪网络

咨询热线:
    0551-63861822
在线客服